In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells

In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells

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High-throughput screening was performed as a phenotypic screening on clinically relevant human mesenchymal stem cells to identify small molecules that affect stem cell fate.From a cga 200 to cga 510 adapter library of pharmacologically active small molecules, we identified kaempferol, which is a natural flavonol, with a high osteogenic activity.Kaempferol achieved the highest levels of bone-specific markers among the chemicals tested in vitro.The osteogenic activity of kaempferol was the same as that of ipriflavone, which is a synthetic isoflavone, in tokidoki hello kitty blind box ovariectomized mouse experiments.Considering that kaempferol is present in various foods such as vegetables and fruits, it will be useful as a new preventive medicine for osteoporosis.